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| Ortega Morales, Benjamín Otto; Santiago Garcia, J.l; Chan Bacab, M.j.; Moppert, Xavier; Miranda Tello, E; Fardeau, M.l.; Carrero, J.c.; Bartolo Perez, P.; Valadez Gonzalez, A; Guezennec, Jean. |
| Aims: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS). Methods and Results: A screening procedure was implemented to detect EPS-producing biofilm bacteria. Isolates MC3B-10 and MC6B-22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B-10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis. Conclusions: Polymer MC3B-10 appears to be a glycoprotein, while EPS MC6B-22 seems to be a true polysaccharide dominated by neutral sugars... |
| Tipo: Text |
Palavras-chave: Tropical intertidal biofilm bacteria; Surfactants; Microbacterium sp; Exopolymers; Bacillus sp; Anionic. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2530.pdf |
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| Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto. |
| A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its... |
| Tipo: Text |
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00171/28260/26530.pdf |
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| Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto. |
| A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its... |
| Tipo: Text |
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00372/48356/48545.pdf |
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| Valero,Nelson; Gómez,Liliana; Pantoja,Manuel; Ramírez,Ramiro. |
| In this paper, the production of humic substances (HS) through the bacterial solubilization of low rank coal (LRC) was evaluated. The evaluation was carried out by 19 bacterial strains isolated in microenvironments with high contents of coal wastes. The biotransformed LRC and the HS produced were quantified in vitro in a liquid growth medium. The humic acids (HA) obtained from the most active bacterial strain were characterized via elemental composition (C, H, N, O), IR analyses, and the E4/E6 ratio; they were then compared with the HA extracted chemically using NaOH. There was LRC biotransformation ranged from 25 to 37%, and HS production ranged from 127 to 3100 mg.L-1. More activity was detected in the isolated strains of Bacillus mycoides,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Low rank coal; Microbacterium sp; Bacillus mycoides; Acinetobacter baumannii; Enterobacter aerogenes. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300021 |
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